In most cases, the goal of ethanol makers is to achieve a higher production associated with alcohol while simultaneously reducing the cost. This desired outcome may be achieved by the use of dual enzymes ethanol production technique using boli brand thermostable alpha-amylase for liquefaction and glucoamylase for saccarification in the medium-temperature cooking phase of ethanol production process. What might cause this to occur? First, let’s take a look at it from the perspective of the process. In order for us to get started, we need to make sure that the following resources are prepared: In the case of maize or cassava, thermostable alpha-amylase boli ha-320 is a member of the enzyme ethanol fermentation, which produces a dark brown liquid with an enzyme activity of 40,000 units per milliliter. glucoamylase boli ga-130, which produces a light to dark brown liquid with an enzyme activity of 150,000 units per milliliter, is also a member of the high-temperature resistant and highly-active dry yeast family. In the event that these items are discovered, the work must begin with the selection of corns and the subsequent disposal of those foreign materials that are not wanted. A hammer mill is used to treat the corn once it has been selected. This mill grinds the corn into flour or a powder that exposes the cornstarch. Additionally, the corn is sieved into certified corn powders that we can easily utilize in phase 2: Enzymes are fuel for ethanol. It is in a slurry tank that the mixing procedure takes place. It is possible to weigh the corn powders before transferring them into the tank. This is done so that the appropriate amount of water may be supplied to accomplish the desired effect. This tank should be filled with centrifuged liquid that is associated with the distiller’s grain at a recycling rate of thirty percent to forty percent. Additionally, thirty percent ammonia or naoh solution should be added to the tank at the same time. The reason for this is to ensure that the ph value on the interior of the liquefaction tank remains within the range of 5.6 to 6.2. In order to liquefy one meter of maize material, it is necessary to add enzymes that produce ethanol at a dose level of around 0.42 to 0.80 kg. We are able to proceed to the subsequent stage, which is liquefaction, at precisely the moment when we are in a position to begin mixing.